Protein_Domain

Part:BBa_K4053001:Design

Designed by: Muhammad Safwan Bin Burhanudin   Group: iGEM21_NTU-Singapore   (2021-10-21)


LBCas12a-RVRR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1201
    Illegal PstI site found at 1699
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1201
    Illegal PstI site found at 1699
    Illegal NotI site found at 3745
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3123
    Illegal XhoI site found at 3754
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1201
    Illegal PstI site found at 1699
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1201
    Illegal PstI site found at 1699
    Illegal NgoMIV site found at 3699
    Illegal NgoMIV site found at 3718
    Illegal AgeI site found at 3427
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References

Tóth, E., Varga, É., Kulcsár, P. I., Kocsis-Jutka, V., Krausz, S. L., Nyeste, A., Welker, Z., Huszár, K., Ligeti, Z., Tálas, A., & Welker, E. (2020). Improved LBCAS12A variants with altered pam specificities further broaden the genome targeting range of Cas12a nucleases. Nucleic Acids Research, 48(7), 3722–3733. https://doi.org/10.1093/nar/gkaa110